|
|
|
Florida Mosquito Control Association |
Arbovirus Surveillance Reports - 2003 November 2003 There were 11 seroconversions to Eastern Equine Encephalitis virus (EEE) for a 0.8% seroconversion rate. This rate is similar to the mean for EEE historical activity for November. There were 124 new HAI Flavivirus positive sentinels (St Louis Encephalitis virus (SLE) antigen used in the HAI assay) confirmed as WN+ by IgM Elisa or Serum Neutralization during November, 2003 for a rate of 9.0%; this is higher than activity levels for last November, but the difference is not significant. The rate of seroconversion (% of birds seroconverting to positive antibody statue for each virus) has been computed, so that comparisons with years past when there were fewer total submissions may be made. Sera from 8 mammals and 2 wild birds were screened by HAI. Antibody to WN was detected in an osprey and a flamingo. Antibody to EEE was also detected in the osprey. Dead animals, primarily birds continue to be submitted for West Nile Virus detection in tissues. During November 2003, tissues from 70 birds and 3 mammals were received for arbovirus detection/isolation. Tissue from a baboon yielded an enterovirus relative. EEE virus was not detected. West Nile virus was detected in 21 birds from:
Of the 262 mosquito pools submitted, one was positive for WN virus:
October 2003 There were 8 seroconversions to Eastern Equine Encephalitis virus (EEE) for a 0.5% seroconversion rate. This rate is similar to the mean for EEE historical activity for October. There were 211 new HAI Flavivirus positive sentinels (St Louis Encephalitis virus (SLE) antigen used in the HAI assay) confirmed as WN+ by IgM Elisa or Serum Neutralization during October, 2003 for a rate of 13.3%; this is similar to activity levels for last October. The rate of seroconversion (% of birds seroconverting to positive antibody statue for each virus) has been computed, so that comparisons with years past when there were fewer total submissions may be made. Sera from 4 mammals and 97 wild birds were screened by HAI. Antibody to WN was detected in one dog and in 7 birds. Antibody to EEE was detected in 4 birds.
Dead animals, primarily birds continue to be submitted for West Nile Virus detection in tissues. During October 2003, tissues from 248 birds and 7 mammals were received for arbovirus detection/isolation. EEE virus was not detected. West Nile virus was detected in 52 birds from:
Of the 810 mosquito pools submitted, one was positive for WN virus:
Collier County submitted 142 pools that had been tested by VecTest during August. There had determined that 4 pools were WN positive, and 11 pools were equivocal positive, with faint bands for both SLE and WN. Molecular assays (TaqMan RT-PCR) for WN virus were performed in our laboratory on all pools and equivocal pools were also tested for SLE. No pools were SLE positive; 1 VecTest WN positive pools was negative by RT-PCR. WN virus was detected in 7 of the VecTest negative pools. Of the equivocal pools, 3 were WN positive by PCR, the remaining 8, were negative. Thus, in this study, if the equivocal test results are considered as positive, VecTest sensitivity was 46%, specificity 93%, predictive value of a positive test (PVP) 40% and predictive value of a negative test (PVN) 94%. If the equivocals are considered negative, the results are 23%, 99%, 75% and 93% respectively. September 2003 There were 12 seroconversions to Eastern Equine Encephalitis virus (EEE) for a 0.6% seroconversion rate. This rate is equal to the mean for EEE historical activity for September. There were 362 new HAI Flavivirus positive sentinels (St Louis Encephalitis virus (SLE) antigen used in the HAI assay) confirmed as WN+ by Serum Neutralization during September, 2003 for a rate of 19.2%; this is similar to activity levels for last September. The rate of seroconversion (% of birds seroconverting to positive antibody statue for each virus) has been computed, so that comparisons with years past when there were fewer total submissions may be made. Sera from 109 wild birds were screened by HAI. Alphavirus (EEE) antibody was present in birds from Okaloosa (3 bluejays, 5 blackbirds), Walton (2 bluejays, 3 cardinal) and Washington (1 blue jays, 1 grackle) counties. Flavivirus antibody was detected in birds from Okaloosa (2 bluejays), Walton (1 cardinal), Orange (1 rail) and Washington (2 cardinals, 1 grackle) counties. Dead animals, primarily birds continue to be submitted for West Nile
Virus detection in tissues. During September 2003, tissues from 344 birds
and 4 mammals were received for arbovirus detection/isolation. EEE virus
was detected in 1 warbler (Walton county). West Nile virus was detected
in 2 horses (marion and Suwannee counties) and in 159 birds from: Bird
data August 2003 There were 40 seroconversions to Eastern Equine Encephalitis virus (EEE) for a 2.2% seroconversion rate. This rate is greater than historical activity for August. Both the numbers and the rate of seroconversion to EEE are still at an all-time high, approaching but not reaching statistical significance. There were 336 new HAI Flavivirus positive sentinels (St Louis Encephalitis virus (SLE) antigen used in the HAI assay) confirmed as WN+ by Serum Neutralization during August, 2003 for a rate of 18.3%; this is similar to activity levels for last August. The rate of seroconversion (% of birds seroconverting to positive antibody statue for each virus) has been computed, so that comparisons with years past when there were fewer total submissions may be made. Sera from 1143 non-sentinel animals (139 wild birds, 4 wild alligators) were screened by HAI. Alphavirus (EEE) antibody was present in 31 birds from Okaloosa (5 bluejays, 5 cardinals, 1 mockingbird), Santa Rosa (3 bluejays, 1 cardinal) and Walton (10 blue jays, 5 cardinals, 1 brown thrasher) counties. Flavivirus antibody was detected in 2 bluejays, Santa Rosa and Walton counties and in 3 wild alligators (Jefferson, Volusia, Bradford counties) Dead animals, primarily birds continue to be submitted for West Nile
Virus detection in tissues. During August 2003, tissues from 458 birds
and 4 mammals were received for arbovirus detection/isolation. EEE virus
was detected in 2 horses (Okechobee and Suwannee counties) and in 2 birds
from Santa Rosa county (finch and dove). West Nile virus was detected
in 199 birds from: Birds
July 2003 There were 126 seroconversions to Eastern Equine Encephalitis virus (EEE); this is significantly greater than historical activity for July. Both the numbers and the rate of seroconversion to EEE are at an all-time high; the rate is higher than that for 1991, an outbreak year, and is significant at the 95% confidence level. There were 79 new HAI Flavivirus positive sentinels (St Louis Encephalitis virus (SLE) antigen used in the HAI assay) confirmed as WN+ by Serum Neutralization during July, 2003; this is similar to activity levels for last July. The rate of seroconversion (% of birds seroconverting to positive antibody statue for each virus) has been computed, so that comparisons with years past when there were fewer total submissions may be made. Sera from 117 non-sentinel animals (115 wild birds, 2 mammals) were screened by HAI. Alphavirus (EEE) antibody was present in 24 birds from Okaloosa (3 cardinals), Santa Rosa (1 cardinal, 4 bluejays), Walton (5 cardinals, 6 blue jays, 1 mockingbird)and Washington (1 cardinals, 2 mockingbirds, 1 sparrow) counties. Flavivirus antibody was detected in 2 birds in Okaloosa (cardinal) and Duval (duck) counties and in a dog from St. Lucie county. Dead animals, primarily birds continue to be submitted for West Nile Virus detection in tissues. During July 2003, tissues from 310 birds and 10 mammals were received for arbovirus detection/isolation. West Nile virus was detected in 57 birds from Alachua (1), Bay (7), Broward(2), Clay (1), Columbia (2), Dade (3), Escambia (19), Hamilton (1), Jackson (3), Levy (5), Madison (2), Nassau (1), Okaloosa (4), Okeechobee (1), Pasco (1), Taylor (3), Washington (1)counties. Bird species included: bluejay (30), crow (18), goose (2), grackle (2), hawk (1), mockingbird (1), shrike (1), and titmouse (1). Additionally EEE was detected in 3 birds from Alachua (cowbird), Leon (finch), Duval (chicken) counties and Highlands J virus was detected in a turkey (Hamilton) and in a dove (Jefferson). Mosquito pools (602) were submitted from Citrus, Collier, Escambia,
Monroe, Palm Beach and St. Johns counties. One pool of Culex nigrapalpus
(1 mosquito) collected in the Florida Keys on 6/24/03 was positive for
West Nile virus. June 2003 The numbers of sera submitted and counties participating in surveillance activities during June were higher than the level of activity for May, as new counties are joining the program. There were 63 seroconversions to Eastern Equine Encephalitis virus (EEE); this is greater than historical activity for June. There were 18 new HAI Flavivirus positive sentinels (St Louis Encephalitis virus (SLE) antigen used in the HAI assay) confirmed as WN+ by Serum Neutralization during June, 2003; this is similar to activity levels for last June. The rate of seroconversion (% of birds seroconverting to positive antibody statue for each virus) has been computed, so that comparisons with years past when there were fewer total submissions may be made. Although numbers of Seroconversions to EEE are at an all-time high, the rate is similar to that for 1991 and 1993, and is approaching significance at the 95% confidence level. Sera from 100 non-sentinel animals (97 wild birds, 3 mammals) were screened by HAI. Alphavirus (EEE) antibody was present in 13 birds from Okaloosa (3 cardinals, 1 bluejay)and Washington (2 cardinals, 8 bluejays) counties. A great horned owl (Orange county) tested positive for antibody to SLE. Dead animals, primarily birds continue to be submitted for West Nile Virus detection in tissues. During June 2003, tissues from 183 birds and 9 mammals were received for arbovirus detection/isolation. West Nile virus was detected in 5 birds from Alachua (crow), Calhoun (bluejay), Columbia (crow), Escambia (dove) and Jackson (crow) counties. EEE was detected in 7 horses (Alachua, Clay, Jefferson (2), Nassau, Orange and Suwanee counties) and 5 birds (Columbia, quail; Hernando, bluejay; Jefferson, finch; Nassau, crow; Taylor, dove). Mosquito pools (927) were submitted from Alachua, Citrus, Escambia,
Gilchrist, Levy, Marion, Monroe and Palm Beach counties. Eastern Equine
Encephalitis virus was isolated (VERO cell culture) from one pool of
35 Oc. infirmatus collected on 6/10/03 in Excambia county. May 2003 The numbers of sera submitted and counties participating in surveillance activities during May were higher than the level of activity for April. Birds are being set out earlier than in previous years and new counties are joining the program. There were 44 seroconversions to Eastern Equine Encephalitis virus (EEE); this is significantly greater than historical activity for May. There were 11 new HAI Flavivirus positive sentinels (St Louis Encephalitis virus (SLE) antigen used in the HAI assay) confirmed as WN+ by Serum Neutralization during May, 2003. Sera from 68 non-sentinel animals (63 wild birds, 3 mammals) were screened by HAI. Alphavirus (EEE) antibody was present in 1 bird (cardinal) from Washington county. Flavivirus HAI antibody was not detected in the birds. Flavivirus HAI antibody, confirmed as West Nile antibody was detected in one horse. Dead animals, primarily birds continue to be submitted for West Nile Virus detection in tissues. During May 2003, tissues from 140 birds and 7 mammals were received for arbovirus detection/isolation. West Nile virus was detected in a crow from Jackson County. EEE was detected in 6 horses (Clay, Columbia, Dixie, Marion, Orange and Pasco counties) and 7 birds (Alachua, pigeon; Lee, dove; Madison, oriole; Santa Rosa, dove, wren, grosbeak; Union, starling). Mosquito pools (189, of which 132 had been assayed previously by VecTest)
were submitted from Escambia, Citrus and Palm Beach counties. No virus
was detected in these pools. April 2003 The numbers of sera submitted and counties participating in surveillance activities during April were higher than the level of activity for March. Birds are being set out earlier than in previous years and new counties are joining the program. There were 15 seroconversions to Eastern Equine Encephalitis virus (EEE); this is significantly higher than historical activity for April. There were 2 new HAI Flavivirus positive sentinels (St Louis Encephalitis virus (SLE) antigen used in the HAI assay) confirmed as WN+ by Serum Neutralization during April, 2003. Sera from 117 non-sentinel animals (95 wild birds, 22 mammals) were screened by HAI. Flavivirus HAI antibody was present in 1 bird (cardinal) from Washington county. Alphavirus (EEE) antibody was present in 7 birds(1 each blackbird, bluejay, cardinal,finch, grackle, sparrow and warbler) from Washington (3), Santa Rosa (2), and Okaloosa (2) counties. Dead animals, primarily birds continue to be submitted for West Nile Virus detection in tissues. During April 2003, tissues from 158 birds and 17 mammals were received for arbovirus detection/isolation. West Nile virus was not been detected in these samples. EEE was detected in 9 horses and 1 cardinal. Mosquito pools (371, of which 132 had been assayed previously by VecTest)
were submitted from Escambia, Monroe, Okaloosa, Palm Beach, Pinellas,
Santa Rosa, Walton and Washington counties. No virus was detected in
these pools There was no report for this page. February 2003 The numbers of sera submitted and counties participating in surveillance activities during February, were similar to the level of activity for January. There were no seroconversions to Eastern Equine Encephalitis virus (EEE); this is not significantly different from historical activity for February. There was 1 new HAI Flavivirus positive sentinel (St Louis Encephalitis virus (SLE) antigen used in the HAI assay) confirmed as WN+ by Serum Neutralization during February, 2003. Sera from 132 non-sentinel animals (110 wild birds, 2 mammals, 20 reptiles) were screened by HAI. Flavivirus HAI antibody was present in 2 birds (1 cardinal, 1 sparrow) from Santa Rosa county. Alphavirus (EEE) antibody was present in 4 birds(1 bluejay, 1 cardinal, 2 sparrows) from Washington (2), Santa Rosa (1), and Okaloosa (1) counties. Dead animals, primarily birds continue to be submitted for West Nile Virus detection in tissues. During February 2003, tissues from 94 birds were received for arbovirus detection/isolation. Virus has not been detected in these samples. Mosquito pools (50, of which 23 had been assayed previously by VecTest)
were submitted from Escambia and Pinellas counties. No virus was detected
in these pools. January 2003 The numbers of sera submitted and counties participating in surveillance activities during January, were lower than the level of activity for December. There were no seroconversions to Eastern Equine Encephalitis virus (EEE); this is not significantly different from historical activity for January. There were 10 new HAI Flavivirus positive sentinels (St Louis Encephalitis virus (SLE) antigen used in the HAI assay) during January, 2003. All were confirmed as WN+ by IgM Elisa or Serum Neutralization. Activity occurred in 8 counties throughout the state. Sera from 196 non-sentinel animals (wild birds and other species) were screened by HAI. Flavivirus HAI antibody was present in 8 birds (3 cardinals, 5 whooping cranes) from Alachua (5), Santa Rosa (1) and Washington (2) counties. Alphavirus (EEE) antibody was present in 21 birds(6 bluejays, 5 cardinals, 5 sparrows,1 whooping crane, 1 dove and 2 secretary birds) from Washington (10), Santa Rosa (7), Alachua (1) and Osceola (2) counties. Dead animals, primarily birds continue to be submitted for West Nile Virus detection in tissues. During January, 2003, tissues from 168 birds, 5 mammals and 1 reptile were received for arbovirus detection/isolation: EEE was detected in 1 warbler collected in November and received in the laboratory on 12/27/02 (Bay county). WNV virus was detected in 3 birds: 1 crow (Leon county) collected 11/21/02 and received 1/7/03, 1 bluejay (Jackson county) collected 12/19/02 and received 1/7/03 and 1 blue jay (Bay county) collected 12/23/02 and received 1/7/03. Mosquito pools (630, of which 97 had been assayed previously by VecTest)
were submitted from Escambia, Monroe, Okaloosa, Orange, Palm Beach, Santa
Rosa, Walton and Washington counties. No virus was detected in these
pools.
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
© 2003
Florida Mosquito Control Association. All rights reserved. |
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||