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Florida Mosquito Control Association |
Arbovirus Surveillance Reports - 2000 January 2000. There was 1 seroconversion to Eastern Equine Encephalitis virus (EEE) during January in Orange county. This is similar to the January average EEE seroconversion rate. There was 1 seroconversion to St Louis Encephalitis virus (SLE) during January, in Osceola countys. This is similar to the average annual SLE seroconversion rate for January. While testing has been considerably reduced during our coldest months, we are preparing for the coming arbovirus season and evaluating procedures for detection of West Nile virus. January sentinel chicken report February 2000. The numbers of sera submitted and counties participating in surveillance activities during February were similar to that for January. In the attached table, seroconversions which are listed as unconfirmed have not yet had a second serum ("rebleed") submitted from that bird. There were no seroconversions to Eastern Equine Encephalitis virus (EEE) or to St Louis Encephalitis virus (SLE) during February. This is similar to the historical average seroconversions. A seroconversion to EEE had occurred in late January in Orange county was confirmed in February. February sentinel chicken report The 1999 annual report totals (Excel file) and graphs (PowerPoint presentation) are available on the web, at www.doh.state.fl.us" - click on Epidemiology, then Veterinary Public Health and Vector-borne Diseases. March 2000. The numbers of sera submitted and counties participating in surveillance activities during March were similar to that for February. In the attached table, seroconversions which are listed as unconfirmed have not yet had a second serum ("rebleed") submitted from that bird. There were 2 seroconversions to Eastern Equine Encephalitis virus (EEE)during March, In Hillsborough and Osceola counties. There were no seroconversions to St Louis Encephalitis virus (SLE) during March. This is similar to the historical average seroconversions. March sentinel chicken report We have performed a retrospective analysis of 81 sentinel chicken sera which had been positive for SLE by the HAI test this past season, using a serum neutralization procedure. The HAI assay is not specific and cross reacting antibody to SLE and West Nile viruses will be seen with that procedure. The serum neutralization assay, however, is the most specific of the serological assays and can distinguish antibody to SLE from that made to West Nile Virus. All sera tested by serum neutralization indicated infection with SLE virus. No neutralizing antibody was found to West Nile virus in these birds. April 2000. The numbers of sera submitted and counties participating in surveillance activities during April were slightly higher than that for March, as some counties have begun setting out flocks for the upcoming season. There were no seroconversions to Eastern Equine Encephalitis virus (EEE) or to St Louis Encephalitis virus (SLE) during our very dry April. This is similar to the historical average seroconversions. April sentinel chicken report. May 2000 The numbers of sera submitted and counties participating in surveillance activities during May were slightly higher than that for April. There was one seroconversion to Eastern Equine Encephalitis virus (EEE) during our very dry May. There were no seroconversions to St Louis Encephalitis virus (SLE). This is similar to the historical average seroconversions for May. Historical May HAI results JUNE 2000 The numbers of sera submitted and counties participating in surveillance activities during June were slightly higher than that for May. There were no seroconversions to Eastern Equine Encephalitis virus (EEE) during our very dry June. This is significantly below the historical level for EEE during June. There were no seroconversions to St Louis Encephalitis virus (SLE). This is similar to the historical average seroconversions for June. Historical HAI results JULY 2000 The numbers of sera submitted and counties participating in surveillance activities during July were higher than those for June. There were no seroconversions to Eastern Equine Encephalitis virus (EEE) during July. This is significantly below the historical level for EEE during July. There was one seroconversion to St. Louis Encephalitis virus (SLE). This is similar to the median historical level of seroconversions for SLE during July. When adequate sample residual is available, sera positive for flavivirus (SLE) in the HAI test are also tested with the chicken IgM ELISA assay for antibody to SLE and to West Nile (WN) virus. No antibody to WN has been detected in Florida birds at this time. Historical HAI results AUGUST 2000 There were three seroconversions to Eastern Equine Encephalitis virus (EEE) during July. This is notably below the historical level for EEE during August. There were 10 seroconversions to St Louis Encephalitis virus (SLE). This is similar to the median historical level of seroconversions for SLE during August. When adequate sample residual is available, sera positive for flavivirus (SLE) in the HAI test are also tested with the chicken IgM ELISA assay for antibody to SLE and to West Nile (WN) virus. No antibody to WN has been detected in FLorida birds at this time. Historical HAI results. SEPTEMBER 2000 Historical HAI results. OCTOBER 2000 The numbers of sera submitted and counties participating in surveillance activities during October were similar to that for September. There were two seroconversions to Eastern Equine Encephalitis virus (EEE) during October. This is similar to the historical level for EEE. There were 80 seroconversions to St Louis Encephalitis virus (SLE). This is similar to the median historical level of seroconversions for SLE during October. When adequate sample residual is available, sera positive for flavivirus (SLE) in the HAI test are also tested with the chicken IgM ELISA assay for antibody to SLE and to West Nile (WN) virus. No antibody to WN has been detected in FLorida birds at this time. Historical HAI results. November 2000 With cooler temperatures, the numbers of sera submitted and counties participating in surveillance activities during November were somewhat less than that for October. There were six seroconversions to Eastern Equine Encephalitis virus (EEE) during November. This is similar to the historical level for EEE. There were 18 seroconversions to St Louis Encephalitis virus (SLE). This is below the median historical level of seroconversions for SLE during November. When adequate sample residual is available, sera positive for flavivirus (SLE) in the HAI test are also tested with the chicken IgM ELISA assay for antibody to SLE and to West Nile (WN) virus. No antibody to WN has been detected in FLorida birds at this time. Historical HAI results. December 2000 With cooler temperatures, the numbers of sera submitted and counties participating in surveillance activities during December were less than that for November. We are preparing an annual summary report of sentinel surveillance for 2000, and will forward it as soon as it is ready. There was one seroconversion to Eastern Equine Encephalitis virus (EEE) during December. This is similar to the median historical level for EEE. There were 3 seroconversions to St Louis Encephalitis virus (SLE). This is similar to the median historical seroconversion level for SLE during December. When adequate sample residual is available, sera positive for flavivirus (SLE) in the HAI test are also tested with the chicken IgM ELISA assay for antibody to SLE and to West Nile (WN) virus. No antibody to WN has been detected in FLorida birds at this time. Historical HAI results  back
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